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Proteinase K Solution, 20mg / ml

Proteinase K

Proteinase K Solution, 20mg / ml

Proteinase K

( RealLine HPV 26 / 53 / 66 ( Str-format

(RealLine Treponema pallidum (Str-format

(Reverase (M-MuLV,RNase H minus

(RealLine Mycoplasma hominis (Str-format

EcoR V from Escherichia coli

‌ برای مشاهده قیمت باید کاربر عضو باشید

 

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كشور سازنده:
آمریکا
نام کارخانه سازنده :
Sigma-Aldrich

 

Properties

Related Categories

3.1.x.x Acting on esters3.x.x.x Hydrolases,Application IndexBiochemicals and Reagents,Enzyme Class Index,

grade   for molecular biology
form   buffered aqueous glycerol solution
concentration   10,000 units/mL
shipped in   wet ice
storage temp.   −20°C

Description

Other Notes

Supplied with 10x Restriction Endonuclease Buffer SB (B8781).

Specificity

Recognition sequence: 5′-GAT/ATC-3′
Ligation and recutting results: After 2-10-fold Eco RV overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >85% of fragments can be ligated, and >95% recut.
Heat inactivation: 80 °C for 20 minutes.

Physical form

Solution in 20 mM Tris-HCl, pH 7.5, 1 mM EDTA, 0.1 mM PMSF, 100 mM NaCl, 10 mM 2-mercaptoethanol, 0.02% polydocanol (v/v), 0.1 mM PMSF, 50% glycerol (v/v) at 4°C

Application

EcoRV is a DNA restriction endonuclease used in molecular biology applications to cleave the recognition site 5′-GAT/ATC-3′, generating DNA fragments with blunt termini.

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