Related Categories |
3.1.x.x Acting on esters, 3.x.x.x Hydrolases,Application Index, Biochemicals and Reagents,Enzyme Class Index, |
grade | for molecular biology |
form | buffered aqueous glycerol solution |
concentration | 10,000 units/mL |
shipped in | wet ice |
storage temp. | −20°C |
Supplied with 10x Restriction Endonuclease Buffer SB (B8781).
Recognition sequence: 5′-GAT/ATC-3′
Ligation and recutting results: After 2-10-fold Eco RV overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >85% of fragments can be ligated, and >95% recut.
Heat inactivation: 80 °C for 20 minutes.
Solution in 20 mM Tris-HCl, pH 7.5, 1 mM EDTA, 0.1 mM PMSF, 100 mM NaCl, 10 mM 2-mercaptoethanol, 0.02% polydocanol (v/v), 0.1 mM PMSF, 50% glycerol (v/v) at 4°C
EcoRV is a DNA restriction endonuclease used in molecular biology applications to cleave the recognition site 5′-GAT/ATC-3′, generating DNA fragments with blunt termini.